apl. Prof. Dr. rer. nat.

Jürgen Pleiss

Principal Investigator (EXC)
Institute of Biochemistry and Technical Biochemistry


+49 711 685-63191


Allmandring 31
70569 Stuttgart
Room: 0.307


  • Bioinformatics and molecular modeling of proteins
  • Enzyme design for biocatalysis

Selected Publications

  1. J. Pleiss. “Thermodynamic activity-based interpretation of enzyme kinetics”. In: Trends Biotechnology 35.5 (2017), pp. 379–382.
  2. P. C. F. Buchholz, C. Vogel, W. Reusch, M. Pohl, D. Rother, A. C. Spieß, and J. Pleiss. “BioCatNet: a database system for the integration of enzyme sequences and biocatalytic experiments”. In: ChemBioChem 17.21 (2016), pp. 2093–2098.
  3. S. Fademrecht, P. N. Scheller, B. M. Nestl, B. Hauer, and J. Pleiss. “Identification of imine reductase-specific sequence motifs”. In: Proteins 84.5 (2016), pp. 600–610.
  4. T. Kulschewski and J. Pleiss. “Binding of solvent molecules to a protein surface in binary mixtures follows a competitive Langmuir model”. In: Langmuir 32.35 (2016), pp. 8960–8968.
  5. A. Taudt, A. Arnold, and J. Pleiss. “Simulation of protein association - Kinetic pathways towards crystal contacts”. In: Physical Review E 91.3 (2015), p. 033311.
  6. C. Vogel and J. Pleiss. “The modular structure of ThDP-dependent enzymes”. In: Proteins 82.10 (2014), pp. 2523–2537.
  7. T. Kulschewski, F. Sasso, F. Secundo, M. Lotti, and J. Pleiss. “Molecular mechanism of deactivation of C. antarctica lipase B by methanol”. In: Journal of Biotechnology 168.4 (2013), pp. 462–469.
  8. A. Seifert, S. Vomund, K. Grohmann, S. Kriening, V. B. Urlacher, S. Laschat, and J. Pleiss. “Rational design of a minimal and highly enriched CYP102A1 mutant library with improved regio-, stereo- and chemoselectivity”. In: ChemBioChem 10.5 (2009), pp. 853–861.
  9. P. Ölschlaeger, S. L. Mayo, and J. Pleiss. “Impact of remote mutations on metallo-betalactamase substrate specificity: implications for the evolution of antibiotic resistance”. In: Protein Science 14.3 (2005), pp. 765–774.
  10. J. Pleiss, M. Fischer, and R. D. Schmid. “Anatomy of lipase binding sites: the scissile fatty acid binding site”. In: Chemistry and Physics of Lipids 93.1 (1998), pp. 67–80.

Working Experience

  • since 2001 Akademischer Oberrat, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
  • 1995–2001 Akademischer Rat, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
  • 1994 Research scientist, Institute of Organic Chemistry, University of Stuttgart
  • 1991–1993 Product Line Manager, Biostructure S.A., Strasbourg
  • 1990 Postdoctoral fellow, Max-Planck-Institute of Biology, Tübingen


Academic Studies and Degrees

  • 2006 Apl.Professor (Bioinformatics and computational biology)
  • 2001 Habilitation, Faculty of Earth and Life Sciences, University of Stuttgart
  • 1990 Dr.rer.nat., University of Tübingen
  • 1987–1990 Doctoral thesis at Max-Planck-Institute of Biology, Tübingen
  • 1986 Diploma in Physics, University of Tübingen
  • 1982–1986 Study of Physics, University of Tübingen
  • 1980–1982 Study of Physics, University of Stuttgart
  • 2003 Offer for a full professorship in bioinformatics at the Technical University of Graz
  • 2003 Merit medal “Science and Technology” of the Socialist Republic of Vietnam
  • Coordinator of a BMBF-funded German-Vietnamese Research and Training Program in bioinformatics and biotechnology (1998–2006) and member of BMBF delegations (2003, 2005)
  • PI of DFG SFB 716 “Particle Simulation” (since 2007) and Member of the Executive Committee (2011–2014)
  • PI of the DFG FOR 1296 “Diversity of Asymmetric Thiamine Catalysis” (since 2010)
  • Steering committee of the annual “European Summer School on Industrial Biotechnology” (since 2015)
  • Organizing committee of the CECAM workshop 1023 “Biological molecules under non-natural conditions” (2014) and 1598 “Proteins in realistic environments: simulation meets experiment” (2018)
  • Evaluator for EU, DFG, BMBF, DAAD, NWO, STW, ANR, FWF
  • Evaluation panel EU-NEST-Pathfinder (2005)
  • Selected Patents (with co-inventors)
    1. Method for the biocatalytic cyclization of terpenes and cyclase employable therein, US2016340666 (2016)
    2. Modified Cytochrome P450 monooxygenases, CY1107811 (2013)
    3. Method for the biocatalytic cyclization of terpenes and cyclase mutants which can be used in said method, EP2640835 (2013)
    4. Cytochrome P450 monooxygenases and their use for oxidizing organic compounds, US7960155 (2011)
    5. Modified lipolytic enzymes and their use EP1130100 (2001)
    6. Cyto-chrome P450 monooxygenase for oxidizing organic compounds, useful especially for converting indole to indigo, has wide substrate range, DE19955605 (2001)
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